ABSTRACT
<p><b>OBJECTIVE</b>To explore a new surgical method and its effect in repairing secondary nasal deformity of unilateral cleft lip.</p><p><b>METHODS</b>Sixty-two patients with secondary unilateral lip and nasal deformity were treated with asymmetric butterfly incision combined with vestibular V-Y advance flap, and the subjective and objective indicators after operation were observed.</p><p><b>RESULTS</b>All incisions healed with primary healing and all cases were followed up for 3-12 months. Fifty-eight cases got symmetric nostril and alar, the sum of absolute value of the nostril differences between both sides < 3 mm. Two cases got symmetric nostril and alar, but the sum of absolute value of the nostril differences between both sides was 3.5 mm and 4.2 mm respectively. The alar relapsed again in 2 cases and the results were not satisfied, the sum of absolute value of the nostril differences between both sides > 5 mm.</p><p><b>CONCLUSIONS</b>The method of using the asymmetric butterfly incision combined with vestibular V-Y mucosa flap can reset the skin,cartilages and mucosa to the anatomic position effectively.</p>
Subject(s)
Animals , Humans , Cartilage , General Surgery , Cleft Lip , General Surgery , Nose , General Surgery , Oral Surgical Procedures , Methods , Rhinoplasty , Methods , Surgical FlapsABSTRACT
This study investigated the protective effect of EGB761 on blood vessels of denervated gastrocnemius of rat and its possible mechanism. Fifteen male adult SD rats were randomly divided into three groups: normal control group (n=3), control group (n=6) and EGB761-treated group (n=6). The rats in the control and EGB761-treated group underwent a neurotomy to bilateral sciatic nerves. Then, they were administered EGB761 [100 mg/(kg·d)] and isovolumic normal saline, respectively by gavage everyday. No treatment was given to the rats in the normal control group. Gastrocnemius was harvested at 1 and 3 week(s) postoperatively in each group. Immunohistochemical method was used to detect the ratio of capillary/fiber (CFR) of denervated gastrocnemius and the expression of VEGF, fetal liver kinase -1(Flk-1) receptor and HSP70 in the vascular wall. The results showed that in the normal control group, VEGF, Flk-1 and HSP70 were expressed in the vessel wall of gastrocnemius, with Flk-1 expressed only in the endothelial cell of vessels. CFR in the EGB761-treated group was significantly higher than that in the control group at 1 week and 3 week(s) after neurotomy. The expression of VEGF and Flk-1 in the vessel wall of both control and EGB761-treated group was much lower than that in the normal control group, and the expression of these proteins in the EGB761-treated group was decreased as compared with that in the control group. The expression of HSP70 in the vessel wall of both control and EGB761-treated groups was enhanced when compared with that in the normal control group, and it was substantially augmented in the EGB761-treated group in comparison to the control group. It was concluded that EGB761 has a protective effect on blood vessels of denervated gastrocnemius, which is related to the increased HSP70 expression but not the expression of VEGF and its receptor Flk-1.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the long-term changes of expanded skin after transplantation.</p><p><b>METHODS</b>The expanded skin in dogs at 0 week, 3 weeks, 6 weeks, 3 months and 6 months after transplantation was observed through areas, histomorphological, electron microscopic, collagen content, biomechanical, and immunohistochemical methods.</p><p><b>RESULTS</b>The expanded skin was close to the control groups in all observations.</p><p><b>CONCLUSION</b>The biological changes after expansion remain in 3 months. The expanded skin will gradually recover to normal in 3-6 months. It is similar to a wound repair process.</p>